Candida albicans Scavenges Host Zinc via Pra1 during Endothelial Invasion
Figure 9
Zrt1 is required for reassociation of soluble Pra1 to the fungal cell.
(A) Predicted three-dimensional structures and interaction of Pra1 and Zrt1. The large extracellular domain of Zrt1 represents the N-terminal tail from amino acids 1–183. (B) C. albicans wild type (M1477), zrt1Δ (M2006) and zrt1Δ+ZRT1 (M2010) were exposed to recombinant His-tagged Pra1 and binding visualised with a fluorescently conjugated anti-His antibody (experiment was performed twice in triplicate); (C) additionally, binding of rPra1 was assessed by Western Blot hybridisation (experiment was performed twice). Note that deletion of ZRT1 precludes reassociation of Pra1 with the fungal cell.