Candida albicans Scavenges Host Zinc via Pra1 during Endothelial Invasion
Figure 6
PRA1 expression is zinc regulated.
C. albicans harbouring GFP under control of either the ACT1 (PACT1-GFP, M135) or PRA1 (PPRA1-GFP, M1522) promoter were incubated in Lee's medium, pH 7.4 either with or without supplementation with (100 µM) zinc. GFP fluorescence was determined at indicated time points and normalised against a C. albicans strain harbouring an empty vector. Note that the addition of zinc fully suppresses expression from the PRA1 promoter. Experiment was performed three times in triplicate.