A High-Resolution View of Genome-Wide Pneumococcal Transformation
Figure 2
Distribution of recombination events across the genome.
(A) Cartoon describing the structure of RSSs as discussed in the text. (B) A detailed view of the RSSs spanning and surrounding the cps locus. The annotation of the region is shown at the top, with the cps locus and flanking pbp genes marked. The red line denotes the extent of the ‘primary locus’ (see text). Underneath, in the panel indicated by the dashed boundary, the RSSs affecting this locus are indicated on the rows by black and grey blocks, as displayed in panel (A). There is a row for each of the 84 transformants, segregated according to the amount of DNA with which they were transformed. (C) Wider view of recombination across the genome. A simplified annotation of the 2,182,009 bp S. pneumoniae 23F-R genome is displayed across the top, with the site of the selected recombination (the cps locus) labelled along with other major chromosomal loci. The RSSs are displayed as indicated in panel (A), with the strains in the same order as in panel (B).