Synergistic Parasite-Pathogen Interactions Mediated by Host Immunity Can Drive the Collapse of Honeybee Colonies
Figure 5
Effect of the down-regulation of the transcription factor dorsal-1A by RNAi on DWV replication in bees.
(A) Dorsal-1A transcript level in bees fed for different times with a sucrose/protein solution, containing dsRNA of honeybee dorsal-1A (dsRNA Dorsal) or dsRNA of Green Fluorescent Protein (dsRNA GFP) as a control. (B) Deformed wing virus genome copies in bees treated as above. The error bars indicate the standard deviation. The significant rate (H = 7.00, df = 1: P = 0.008) of silencing of the target gene triggered a significant increase (H = 9.61, df = 1: P = 0.002) of viral replication.