Plasticity of the β-Trefoil Protein Fold in the Recognition and Control of Invertebrate Predators and Parasites by a Fungal Defence System
Figure 2
Carbohydrate-binding specificity of CCL2.
Fluorescently labeled CCL2 was analyzed for binding to the mammalian glycan array (V3.1) of the Consortium for Functional Glycomics (CFG). Results shown are averages of triplicate measurements of fluorescence intensity at a lectin concentration of 200 µg/ml. Error bars indicate the standard deviations of the mean. Glycan structures are depicted for those epitopes with highest relative fluorescence. The raw data and the entire list of glycans with the respective spacers can be found on the CFG homepage [http://functionalglycomics.org/] or in Tables S2 and S3. Binding of 6'sulfo-sialyllactose (glycan #45) is likely to be an artifact since it is also bound by fucose-binding lectin AAL [http://functionalglycomics.org/].