Nitazoxanide Stimulates Autophagy and Inhibits mTORC1 Signaling and Intracellular Proliferation of Mycobacterium tuberculosis
Figure 2
Increased EGFP-LC3 processing and inhibition of mTORC1 signaling by NTZ and TIZ.
Cells were treated with the indicated concentrations of NTZ, TIZ or rapamycin for 4 h (A) or 24 h (B). In panel C, cells were treated with 10 µM NTZ, 10 µM TIZ, 30 nM rapamycin, or DMSO without or with 0.1 µM bafilomycin A1 for 4 h. EGFP-LC3 processing was examined by immunoblotting with antibodies against GFP, mTORC1 activity using antisera against phospho-S6K Thr389 and total S6K, and mTORC2 activity with antisera against phospho-AKT Ser473 and total AKT. Total AKT was also used as a loading control.