Preferential Entry of Botulinum Neurotoxin A Hc Domain through Intestinal Crypt Cells and Targeting to Cholinergic Neurons of the Mouse Intestine
Figure 2
Binding of HcA to frozen sections of the mouse upper small intestine.
(A) Sections were overlaid with Alexa488-HcA and were counterstained with TRITC-Phalloidin. HcA staining (green) was observed in intestinal crypts, small cells in intestinal villi beneath the enterocytes, as well as in filaments from the submucosa. (B) Counterstaining with TRITC-UEA1 (red) labeling of Paneth cells in intestinal crypts and small cells in intestinal villi. (C) Counterstaining with anti-neurofilament (NF) antibodies and co-labelling (arrowhead) of neurofilaments with HcA in the submucosa. (scale bars = 50 µm).