Calcium Influx Rescues Adenylate Cyclase-Hemolysin from Rapid Cell Membrane Removal and Enables Phagocyte Permeabilization by Toxin Pores
Figure 9
Potassium efflux delays endocytosis of dCyaA toxoid.
A) J774A.1 cells were incubated for 20 minutes at 37°C with 5 µg/ml of Alexa 488-labeled dCyaA in HBSS containing 5 mM KCl or in HBSS containing 50 mM KCl. Uptake of dCyaA into cells was analyzed by live cell imaging and (B) numbers of endosomes in cell cytoplasm were quantified (n = 3).