The Invasive Capacity of HPV Transformed Cells Requires the hDlg-Dependent Enhancement of SGEF/RhoG Activity
Figure 5
Dlg enhances RhoG activity in an SGEF dependent manner.
Panel A. HEK293 cells were either transfected with vector or HA-tagged Dlg and Flag-tagged SGEF as indicated. After 24 hrs cell extracts were made which were then incubated with purified GST-ELMO to pull down active RhoG which was detected by western blot analysis. The three lower panels show total protein inputs for RhoG, Dlg and SGEF. Panel B. Graph showing the quantification from multiple GST-ELMO pull-downs, showing the fold change in the levels of RhoG activity under the different experimental conditions. Error bars represent ±SD of three independent experiments Panel C. Extracts from HaCaT cells stably ablated for hDlg expression (shDlg) either with or without Dlg rescue expression were used in a GST-ELMO pulldown assay to determine RhoG activity. Extracts from untreated HaCaT cells or HaCaT cells stably expressing control shRNA(TR2) were used as control.