Biochemical Properties of Highly Neuroinvasive Prion Strains
Figure 5
Soluble to insoluble PrP ratio varies depending on the prion strain.
(A) Brain homogenate was ultracentrifuged, and the supernatant and pellet fractions were subjected to SDS-PAGE and immunoblotting for PrP. The strongly neuroinvasive strain 22 L has significantly higher levels of insoluble PrP as compared to the weakly neuroinvasive 87 V. (B) Plot of the percentage of insoluble PrP. S = supernatant, P = pellet. ** indicates a P value of <0.001 for 22 L as compared with 87 V.