Multifaceted Regulation of Translational Readthrough by RNA Replication Elements in a Tombusvirus
Figure 5
Restoring RT and genome replication with a heterologous RT sequence.
(A) At the top, a portion of the SL-PRTE structure is shown with the insertion site (arrow) of wt and mutant forms of the 6 nt long TMV RTE sequence. Wt and mutant TMV RTE sequences in corresponding genomic mutants are shown below. Substitutions in the mutant TMV RTE are underlined. (B) In vitro translation in wge of CIRV genomes containing mutations disrupting the PRTE-DRTE interaction (B2 and BS2) as well as the wt TMV RTE (B2T and BS2T) or the mutated TMV RTE (B2TN and BS2TN). (C) Northern blot analysis of wt and mutant CIRV genomes in protoplasts and quantification of plus-strand viral genome accumulation.