Follicular Dendritic Cell-Specific Prion Protein (PrPc) Expression Alone Is Sufficient to Sustain Prion Infection in the Spleen
Figure 7
Effect of FDC-restricted PrPC-ablation on immune complex trapping.
A) Mice were passively immunized with preformed PAP immune complexes, and 24 h later, the presence of immune complexes (red) upon FDC (CD35+ cells, green) assessed by IHC. Scale bar, 100 µm. B) Morphometric analysis confirmed that the magnitude of the immune complex-trapping co-localized upon the surfaces of FDC from Prnp-ablated Prnpflox/-→CD21-Cre Prnpflox/- mice was not significantly different from that observed in spleens from control mice. This analysis also confirmed that the immune complexes were preferentially associated with FDC in these tissues and significantly greater than the null hypothesis that the pixels were randomly distributed. *, P<1×10−21; **, P<1×10−32; *** P<9×10−28; n = 40 FDC networks/group. For all panels n = 6 mice/group.