Two Novel Transcriptional Regulators Are Essential for Infection-related Morphogenesis and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae
Figure 9
MoSom1 interacts with MoStu1, MoCdtf1 and CpkA in M. oryzae.
(A) Strong interaction between MoSom1 and MoStu1 (an APSES protein) and MoCdtf1 in yeast two-hybrid assays. The Leu+ and Trp+ yeast transformants were assayed for growth on SD-Trp-Leu-His-Ade medium at specified concentrations 1×105, 1×104, 1×103, 1×102 and 10 cells each 10 µl droplet. (B) Weak interaction between MoSom1 and CpkA in a yeast two-hybrid assay with the presence of 5 mM cAMP. Transformants were tested for growth on SD-Trp-Leu-His-Ade medium with or without 5 mM cAMP.