Two Novel Transcriptional Regulators Are Essential for Infection-related Morphogenesis and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae
Figure 5
Both Δmosom1 and Δmocdtf1 mutants show an easily wettable phenotype.
(A) Surface hydrophobicity of the wild type strain Guy11 and the mutants SK27 (Δmosom1) and CTK15 (Δmocdtf1) was assessed by placing a 10 µl drop water, 250 µg/ml tween20, 0.2% SDS+50 mM EDTA, and 0.2% gelatin on the 7-day-old cultures, respectively. Drops of water and 0.2% gelatin remained on the cultures of Guy11 and the old mycelium of the mutant CTK15, while the others were soaked into colonies. The photographs were taken after 12 h incubation. (B) Expression of the genes coding hydrophobins in the Δmosom1 (SK27) and Δmocdtf1 (CTK15) mutants measured by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Error bars represent the standard deviation. Asterisks indicate significant difference at P = 0.01.