Neonatal CD8 T-cell Hierarchy Is Distinct from Adults and Is Influenced by Intrinsic T cell Properties in Respiratory Syncytial Virus Infected Mice
Figure 5
TCR Vβ screening of naïve and epitope-specific CD8+ T cells in adult and neonatal mice.
(A) TCR Vβ antibody screen of CD8+ T cells from the spleen of naïve adult and naïve neonatal CB6F1 mice. Samples were stained with surface phenotype antibodies, and a panel of FITC-labeled Vβ-specific antibodies. (B) TCR Vβ antibody screen of epitope-specific (KdM282-90 and DbM187-195) CD8+ T cell populations in the lungs of RSV-infected adults and neonates. Lung lymphocytes were stained with surface phenotype antibodies, epitope-specific tetramer, and Vβ-specific antibodies. All TRBV regions are listed using IMGT nomenclature. Data represents two independent experiments performed with pooled naïve splenic or lung lymphocytes.