Drosophila melanogaster as an Animal Model for the Study of Pseudomonas aeruginosa Biofilm Infections In Vivo
Figure 2
In vivo P. aeruginosa biofilms stain positively for EPS and DNA.
(A) Red autofluorescence, (B) green autofluorescence, (C) DAPI-stained nuclei (all indicated by grey arrow) and (D) merge image of uninfected Drosophila crop. (E) Aggregative red fluorescent PAO1pCHAP6656 (white arrow) along with red autofluorescence (grey arrow), (F) green fluorescent EPS (white arrow) staining and autofluorescence (grey arrow), (G) DAPI staining of bacteria (white arrow) and Drosophila nuclei (grey arrow) and (H) merge image of PAO1-infected crops. DNAse and cellulase treatment of P. aeruginosa-infected crops. (I) Non-aggregative red fluorescent PAO1pCHAP6656 (white arrow) along with red autofluorescence (grey arrow), (J) autofluorescence (grey arrow) and lack of EPS staining with FITC-labeled HHA lectin. (K) DAPI staining of Drosophila nuclei (grey arrow) and absence of bacterial DNA staining and (L) merge image of DNAse and cellulase treated PAO1 in infected crops (white arrow). Scale bars in indicate 100 µM. At least three infected crops were examined from two separate infections and representative images are shown.