Fitness Landscape of Antibiotic Tolerance in Pseudomonas aeruginosa Biofilms
Figure 3
Fitness characterization of candidate mutants in different physiological states.
(A, B) Competitions were started with equal amounts of mutant and reference cells. The y-axis indicates the relative count of mutants over the reference strain following one round of the indicated experimental challenge (as explained in Materials and Methods). As cultures undergo different numbers of generations during each type of challenge, values from different challenges for the same mutant are not directly comparable. Error bars indicate the standard error of at least 8, 4, and 3 experiments for the Bio-TOB, Bio-ND, and Pla-ND conditions, respectively. Mutants in (A) have an advantage over wild-type in the Bio-TOB competition; mutants in (B) have a disadvantage. Gene annotations, which were updated from the original genome annotation [48] by BLAST comparisons [14] against the NCBI non-redundant database, are in Table S3. (C) Growth curves for representative strains from panels (A) and (B) with 4 µg/ml tobramycin are shown.