The Role of Sialyl Glycan Recognition in Host Tissue Tropism of the Avian Parasite Eimeria tenella
Figure 2
Details of localization of EtMIC3 and EtAMA1 in invading parasites.
(a) Immunofluorescence localisation (left, top and bottom panels) and DIC (right, top and bottom panels) of EtMIC3 (green) and EtAMA1 (red) in fixed and permeabilised invading E. tenella sporozoites on MDBK monolayers. Blue counterstain is DAPI. Both EtMIC3 and EtAMA1 label necklace- like structures present at the junction between the invading parasite and the host cell; the labelling is closely associated but does not co-localize. The top panels indicate the position of the parasite-host junction and the bottom panels give a clear cross sectional image of the non-overlapping staining patterns. The bar represents 2 µm in top panels, 1 µm in bottom panels. (b) Immunofluorescence localisation (left panel) of EtMIC3 (red) and EtAMA1 (green) in fixed, unpermeabilized invading E. tenella sporozoite (left image) and combined with DIC (right panel). The parasite is invading from left to right and EtMIC3 is found on the surface at the region of the moving junction (arrowheads) and as a trail on the host cell surface. EtAMA1 was not detected. Blue counterstain is DAPI, N indicates parasite nucleus. The bar represents 2 µm. (c) Immunofluorescent localization of EtMIC3 (green) and EtMIC5 (red) in permeabilised E. tenella sporozoite attached to MDBK cell in culture. EtMIC3 is concentrated at the extended conoid and the apical surface of the sporozoite whereas EtMIC5 is slightly posterior to this region. The bar represents 3 µm.