Endophytic Life Strategies Decoded by Genome and Transcriptome Analyses of the Mutualistic Root Symbiont Piriformospora indica
Figure 2
Biotrophic growth of P. indica in barley root cortex cells.
Living root cortex cell with fungal hyphae inside (4 dpi). Fungal structures were stained with WGA-AF488 (green), plant membranes were stained with the endocytosis marker FM4-64 (red). A) FM4-64 and WGA-AF488, B) bright field, C) overlay. In contrast to extracellular hyphae, intracellular hyphae were not stainable with WGA-AF488, indicating that the hyphae remained enveloped in a plant-derived membrane throughout intracellular growth. Internalization of FM4-64 in the form of endomembrane structures after 20 min of incubation are visible inside the plant cell, indicating viability of the cells. Images were taken with a CLM, Leica TCS SP5 (Leica, Bensheim, Germany). The bar represents 10 µm.