Chlamydia trachomatis Co-opts the FGF2 Signaling Pathway to Enhance Infection
Figure 5
C. trachomatis L2 infection stimulates FGF2 expression, production, and release.
(A) Total RNA was isolated from the HeLa cells infected with C. trachomatis L2 for indicated time. fgf2 mRNA was assessed by qRT-PCR and normalized to gapdh mRNA. (B) Total FGF2 protein (cell associated and secreted) was measured by Quantikine kit and normalized to uninfected cells (UI) and normalized for total cell number by quantifying total LDH activity. (C) Cell lysates (upper panel) and cell supernatants (lower panel) of FGF2 were collected from HeLa cells infected with C. trachomatis L2 for the indicated times. FGF2 was detected by immunoblot (upper panel) or by immunoprecipitation (lower panel). GAPDH serves as a loading control.