Chlamydia trachomatis Co-opts the FGF2 Signaling Pathway to Enhance Infection
Figure 2
FGF2 co-localizes with cell surface bound EBs.
HeLa cells were infected for 1 hr with C. trachomatis L2 in SFM supplemented with FGF1 (left panels; 100 ng/mL) or with FGF2 (right panels; 100 ng/mL). (A) Cells were fixed and stained with FGF1 antibody (left panels) or FGF2 antibody (right panels). Cell surface associated EBs and host cell nuclei were visualized by DAPI (blue) staining. Arrows point to EBs. (B) Co-localization of EBs with FGF1 or FGF2 was quantified from at least 8 different fields. The data are expressed as a mean percentage of FGF-associated bacteria (± SEM) compared to total bacteria. *** p<0.001