Structure of Herpes Simplex Virus Glycoprotein D Bound to the Human Receptor Nectin-1
Figure 5
Unliganded gD and gD/receptor complexes.
A, B. Surface representation of gD bound to nectin-1, with nectin-1 removed (A) or present (B). Residues 23–38 of gD are colored light green, the V-domain (residues 56–185) is colored yellow and the other residues from N-terminal (residues 39–55) and C-terminal (residues 186–250) extensions are in gray. Residues buried by nectin-1 in the gD/Nectin-1 complex are colored in dark blue. The V domain of nectin-1 is colored violet and the C1 domain is pink. C, D. View of gD bound to HVEM with HVEM removed (C) or present (D). Colored as in panel A with the N-terminal region involved in HVEM binding colored in dark green (residues 1 to 16) and in light-green (residues 17–38). Residues 1–16 of gD, which were not localized in the gD/Nectin-1 structure, fold back to form a hairpin structure when HVEM is bound. In the latter conformation, these residues mask the nectin-1 binding site. HVEM is shown in light blue. E. Conformation of unliganded gD colored as in panel A with residues 260–306 from the C-terminal extension colored in red.