Structure of Herpes Simplex Virus Glycoprotein D Bound to the Human Receptor Nectin-1
Figure 4
Effect of nectin-1 mutations on HSV entry. A
. Receptor negative B78H1 cells were transfected with full-length nectin-1 carrying the indicated mutations. HSV KOS-tk12 virus was added at various MOI. Entry was recorded 6h post infection by measuring β-galactosidase activity. A representative experiment is shown. Background signal from mock-transfected cells is also shown. B. Summary of all the entry data at an MOI of 5 pfu/cell. Levels of entry into cells expressing nectin-1 mutants are reported as the percentage of entry into B78H1 cells transfected with wt nectin-1. An average of 6 experiments is shown with standard deviation.