Structure of the Vesicular Stomatitis Virus N0-P Complex
Figure 6
The N-terminal and C-terminal region of P60 flanking the MoRE exhibits conformational flexibility in the soluble complex.
(A) Comparison of the 2D 1H-15N HSQC NMR spectra of free 15N, 13C, 2H-labelled P60 (blue) and in complex with NΔ21 (red). Both spectra were recorded at 14.1 T and 25°C in 20 mM Tris-HCl, 150 mM NaCl, 50 mM Glu, 50 mM Arg with 10% D2O adjusted to pH 6.0. The labels indicate the assignment of the resonances of the complex. (B) 15N R2 spin relaxation rates measured under the same conditions as (A) for the free 15N-labeled P60 (blue) and the NΔ210-P60 complex (red). (C) 15N R2 spin relaxation rates measured at 14.1 T and 10°C of the free 15N-labeled P60 (blue), a mixture of 0.27 mM 15N-labeled P60 and 0.09 mM unlabeled MBP-NΔ21 (green) and a mixture of 0.24 mM 15N-labeled P60 and 0.17 mM unlabeled MBP-NΔ21 (red).