Envelope Deglycosylation Enhances Antigenicity of HIV-1 gp41 Epitopes for Both Broad Neutralizing Antibodies and Their Unmutated Ancestor Antibodies
Figure 5
Binding of WT glycosylated and deglycosylated JRFL gp140 Env on DC-SIGN-expressing NIH 3T3 cells.
The binding of AF647-labeled WT glycosylated (histogram in red lines and deglycosylated JRFL Env gp140 (histograms in blue lines) to parent NIH 3T3 (histograms shaded) and DC-SIGN-expressing NIH 3T3 cells (histograms not shaded) were analyzed by flow cytometry. Data shown are representative of three experiments.