Histo-Blood Group Antigens Act as Attachment Factors of Rabbit Hemorrhagic Disease Virus Infection in a Virus Strain-Dependent Manner
Figure 4
Immunohistochemistry of A, B and H expression and RHDV binding to duodenum and trachea.
Staining of the duodenum, trachea and liver of A+B+ rabbits by an anti-A, an anti-B and the H type 2 specific UEA-I lectin. Binding of RHDV (shown a G3 strain) was performed by incubating liver extracts on tissue sections followed by detection using an anti-RHDV monoclonal antibody as described in the “materials and methods” section. Negative controls correspond to section where the liver extracts were omitted. The simple bar corresponds to 100 µm and the double bar to 200 µm. A 4x higher magnification is inserted into the RHDV/duodenum picture, showing the labeling of the crypt epithelial cells. The white star is the Lieberkühn crypts (surface layer of the duodenal mucosa). The black star is the Brünners' glands (deep layer of the duodenal mucosa). The arrow indicates the epithelial layer of the trachea. The triangle indicates the liver parenchyma.