The Steroid Catabolic Pathway of the Intracellular Pathogen Rhodococcus equi Is Important for Pathogenesis and a Target for Vaccine Development
Figure 3
Gas chromatography profiles showing the formation of methylhexahydroindanone propionate intermediates during whole cell biotransformations of 4-androstene-3,17-dione (AD) by mutant strains of R. equi RE1 at T = 120 hours.
Methylhexahydroindane-1,5-dione propionate (HIP) and 5-hydroxy-methylhexahydro-1-indanone propionate (5OH-HIP) accumulation in cell cultures of RE1ΔipdABΔipdA2B2 and RE1ΔfadE30, respectively. No accumulation is observed in cell cultures of RE1ΔipdAB. Lower panel shows the GC profiles of HIP (200 mg/L) and 5OH-HIP (50 mg/L) as authentic samples. Abbreviations: 1,4-androstadiene-3,17-dione (ADD), 3-hydroxy-9,10-secoandrost-1,3,5(10)-triene-9,17-dione (3-HSA), progesterone (50 mg/L) internal standard (I.S.).