The Steroid Catabolic Pathway of the Intracellular Pathogen Rhodococcus equi Is Important for Pathogenesis and a Target for Vaccine Development
Figure 2
Growth curves of wild type, mutant and complemented mutant strains of R. equi RE1 in mineral medium supplemented with 4-androstene-3,17-dione (AD) or 3aα-H-4α(3′-propionic acid)-5α-hydroxy-7aβ-methylhexahydro-1-indanone (5OH-HIP) as a sole carbon and energy source.
Panels A and B show growth curves of wild type strain (diamonds) and mutants strains RE1ΔipdAB (squares), RE1ΔipdA2B2 (triangles), RE1ΔipdABΔipdA2B2 (crosses), RE1ΔfadE30 (asterisks) and RE1ΔfadA6 (circles) in MM+AD and MM+5OH-HIP, respectively. Panels C and D show growth curves of complemented strains of RE1ΔipdAB (diamonds) and RE1ΔfadE30 (squares) in MM+AD and MM+5OH-HIP, respectively. Curves represent averages of two independent experiments. Error bars represent standard deviations. Media with AD are turbid; therefore culture protein content was measured instead of optical density.