Crystallography of a Lewis-Binding Norovirus, Elucidation of Strain-Specificity to the Polymorphic Human Histo-Blood Group Antigens
Figure 9
The structures of the type I and type II disaccharide precursors of HBGAs and their possible steric locations interacting with the VA207 P dimer.
The type I (A) and type II (B) disaccharide precursors are shown in stereo view. The two protomers are shown in cyan and purple, respectively and atoms are colored in grey (C), red (O), and blue (N). The –CH3OH group of the GlcNAc of the type I disaccharide collides (1.3 Å) with the side chain of S439 (A, right, marked by a blue arrow), making the Leb sterically difficult to bind to the wild type VA207. R indicates the position that links to backbone. Blue dashed lines in panel B indicate the hydrogen bonds between GlcNAc and Y389/S439 in complex structure. The two fucose residues representing H and Lewis epitopes are omitted for clarity. Gal, galactose; GlcNAc, N-acetylglucosamine.