Multiple Translocation of the AVR-Pita Effector Gene among Chromosomes of the Rice Blast Fungus Magnaporthe oryzae and Related Species
Figure 9
CHEF-Southern analyses of chromosomal locations of AVR-Pita3 in representative isolates of P. oryzae.
(A) Chromosomal DNAs separated in the contour-clamped homogeneous electric field (CHEF). The CHEF gel was stained with ethidium bromide. Note that the left five samples did not run straight. (B) Chromosomal bands carrying AVR-Pita3. The chromosomal DNAs in (A) were blotted and hybridized with the AVR-Pita3 probe (APita3-958). (C) Identification of the chromosomes hybridizing to the AVR-Pita3 probe. Chromosomes on the gel (A) were identified by reprobing the membrane (B) with chromosome – specific markers; e.g., T1-A11 and CH5-75H for chromosome 7 and T1-G4 for chromosome 6. Chromosomal bands that hybridized to the AVR-Pita3 probe in (B) were painted with the chromosome-specific colors used in Figure 5A.