Multiple Translocation of the AVR-Pita Effector Gene among Chromosomes of the Rice Blast Fungus Magnaporthe oryzae and Related Species
Figure 8
Functional analysis of AVR-Pita homologs with respect to avirulence activity.
(A) Pathogenicity of Oryza isolate P-2b (O-8J) and its transformants expressing various AVR-Pita homologs (Y36 through Y1) on rice cultivars, Shin2 (S2) carrying pita and Yashiromochi (YM) carrying Pita, 7 days after inoculation. See Table 1 for details of the transgenes. (B) Alignment of amino acid sequences of the AVR-Pita homologs. DQ855958 is the accession number of AVR-Pita3 [39] used as an outgroup in Figure 7A. Functional (triggers Pita-mediated resistance) and non-functional (fails to trigger Pita-mediated resistance) homologs are shown in red and green, respectively. Amino acids shown in blue letters indicate substitutions shared by the three non-functional J1 homologs in comparison with the functional J2(O-29J) and those shared by the two non-functional PO homologs in comparison with the functional PO (4224-7-8).