Multiple Translocation of the AVR-Pita Effector Gene among Chromosomes of the Rice Blast Fungus Magnaporthe oryzae and Related Species
Figure 5
Frequent AVR-Pita translocation occurred independently from major chromosomal translocations or duplications.
(A) A diagram of electrophoretic karyotypes of Pyricularia isolates revealed by Southern analyses. Blots of CHEF gels were hybridized with the markers enclosed in colored rectangles in Figure 4A. Chromosomal bands that hybridized exclusively to markers assigned to a single, same chromosome are painted in the color assigned to the chromosome in Figure 4. Chromosomal bands that hybridized to markers assigned to two or more, different chromosomes are divided with vertical lines and painted in the colors assigned to those chromosomes. Chromosomal bands that were smaller than the average size of chromosome 7 and did not hybridize to any chromosome-specific probes were considered to be supernumerary chromosomes and are painted in black. Asterisks indicate isolates which are deduced to have suffered from chromosomal rearrangements such as translocations or duplications. (B) A diagram of chromosomal locations of AVR-Pita homologs. Chromosomal bands that hybridized to the AVR-Pita probe (APita766) were painted with the chromosome-specific colors used in (A). The RFLP types defined in Figure 1 are shown above isolate codes. Hyphens indicate isolates carrying no AVR-Pita homologs that are detectable in the genomic Southern analysis. Shaded isolates are representatives chosen for further analyses of AVR-Pita flanks (see Figure 6). Isolates from Eleusine, Triticum, Lolium, Brachiaria, Eragrostis, and Leersia are omitted from this diagram because all isolates from these hosts are non-carriers of AVR-Pita homologs (see Figure S1).