Multiple Translocation of the AVR-Pita Effector Gene among Chromosomes of the Rice Blast Fungus Magnaporthe oryzae and Related Species
Figure 4
Identification of chromosome specific markers spanning the P. oryzae chromosomes.
(A) A genetic map of a cross between P. oryzae isolates, Si-1J and T-4B. Asterisks indicate chromosome-specific markers reported by Nitta et al. [47]. Double asterisks indicate chromosome-specific SSR markers reported by Zheng et al. [52]. Markers prefixed with “Tel” are telomere signals produced by Southern hybridization with telomere repeats (TTAGGG)10. Letters at the end of the markers (s and t) represent parents they are derived from (s, from Si-1J; t, from T-4B). Markers in red, yellow, brown, green, light blue, blue, and violet, were used in the CHEF-Southern analysis (B) for identification of chromosomes 1, 2, 3, 4, 5, 6, and 7, respectively. Markers enclosed in rectangles were used for karyotype analysis in Figure 5A. (B) Identification of homologous chromosomes in the parental isolates (Si-1J and T-4B) on a CHEF gel. Chromosomal DNAs in the parental isolates were separated on a CHEF gel and stained with ethidium bromide (the left panel). Sizes of Schizosaccharomyces pombe chromosomes are indicated on the left of the gel photograph. The gel was then blotted and probed with the RFLP markers shown in the chromosome-specific colors in (A). Seven hybridization patterns (S1T1 through S6T5) are shown in the middle seven panels with marker names on the top as examples. Based on these Southern analyses, chromosome number(s) were assigned to each band (the right panel). The diagrammatic chromosomal bands are painted in the chromosome-specific colors used in (A). S (painted in black) indicates a supernumerary chromosome described previously [48].