Multiple Translocation of the AVR-Pita Effector Gene among Chromosomes of the Rice Blast Fungus Magnaporthe oryzae and Related Species
Figure 3
CHEF-Southern analyses of chromosomal locations of AVR-Pita homologs in Pyricularia isolates.
(A) Representative examples of chromosomal DNAs separated by contour-clamped homogeneous electric field (CHEF) gel electrophoresis. White arrowheads indicate chromosomal bands that hybridized to the AVR-Pita probe (APita766) in (B). Black arrowheads indicate chromosomal bands that hybridized to a chromosome 7 – specific cosmid marker, T1A11 (see Figure 4A), in (C). (B) Chromosomal bands carrying AVR-Pita homologs. The chromosomal DNAs in (A) were blotted and hybridized with the AVR-Pita probe (APita766). (C) The location of chromosome 7. The membrane in (B) was reprobed with the chromosome 7 – specific cosmid marker, T1A11.