Multiple Translocation of the AVR-Pita Effector Gene among Chromosomes of the Rice Blast Fungus Magnaporthe oryzae and Related Species
Figure 2
Linkage analyses of AVR-Pita homologs.
(A) A map of chromosome 4 constructed by using an F1 population derived from a cross between O-29J and O-30C. Segregation data of three AVR-Pita homologs (AVR-Pita-J1(O-29J), AVR-Pita-J2(O-29J), and AVR-Pita-CH(O-30C), enclosed in black rectangles) and the avirulence on cv. Yashiro-mochi (Pita carrier) determined by infection assay (AvrPita, enclosed in an open oval) were combined with those of genetic markers reported by Luo et al. [46]. A chromosome 4 – specific marker is indicated by an asterisk. “Tell” is a telomere signal produced by Southern hybridization with a telomere repeat oligonucleotide (TTAGGG)10. (B) A map of chromosome 6 constructed by using an F1 population derived from a cross between Si-6I and T-7B. An AVR-Pita homolog found in Si-6I is enclosed in a black rectangle. Chromosome 6 – specific markers [47] are indicated by asterisks. Markers prefixed with “Tel” are telomere signals produced by Southern hybridization with the telomere repeat probe. Letters at the end of the markers (s and t) represent parents they are derived from. For example, CH4-121H-st represents two cosegregating fragments, one from Si-6I (Setaria isolate) and one from T-7B (Triticum isolate). (C) Southern blot analysis of an F1 population derived from a cross between O-23IN and T-4B. Genomic DNAs representing each meiotic product from ten tetrads (Set41 through Set54) were digested with BamHI and hybridized with telomere repeat (upper panel) and AVR-Pita (lower panel) probes. Open arrowheads indicate restriction fragments inherited in a non-Mendelian manner.