The Trypanosoma cruzi Protease Cruzain Mediates Immune Evasion
Figure 6
Proteolytic cleavage of r-human NF-κB P65 by wild type- and r-cruzain but not by proteases expressed by cruzain-deficient T. cruzi.
Recombinant human NF-κB P65 was treated or not with cruzain or parasite extracts. Lane 1, untreated control; lane 2, r-cruzain (1∶1 molar ratio); lanes 3–4: dilutions 1∶10 and 1∶100 of r-cruzain, respectively; lane 5, r-cruzain (1∶1 molar ratio) and 10 µM K11777; lane 6, WT cruzain; lane 7, proteases in cruzain-deficient T. cruzi. Results are representative of two independent experiments.