The Trypanosoma cruzi Protease Cruzain Mediates Immune Evasion
Figure 5
Colocalization of cruzain and NF-κB P65.
A–C. Representative examples of colocalization of labeled cruzain (A) (red fluorescence) and NF-κB P65 (B) (green fluorescence) in the flagellar pocket region and/or cell surface of interiorized transforming parasites (C, merge). D. Lack of colocalization of accumulated cruzain (red fluorescence) and NF-κB P65 (green) in a macrophage infected as above with WT T. cruzi and treated with 10 µM K11777 that induces accumulation of unprocessed zymogen in the Golgi compartment (arrow) (22).