The Trypanosoma cruzi Protease Cruzain Mediates Immune Evasion
Figure 4
NF-κB P65 localizes to the cell surface of wild type parasites.
Localization of NF-κB P65 (green fluorescence) was investigated in mouse macrophages. Parasite and host cell DNA was labeled with PI (red). Non-infected macrophages showed an evenly scattered distribution of cytoplasmic NF-κB P65 (E). Intracellular WT- T. cruzi appeared coated with macrophage NF-κB P65 (A, B). In contrast, abundant cytoplasmic (green fluorescence) and nuclear (yellow fluorescence) NF-κB P65 occurred in macrophages infected with cruzain deficient T. cruzi (C–D). Macrophages were infected for 30 min (A, C), and 60 min (B, D). Results are representative of four independent experiments.