The Trypanosoma cruzi Protease Cruzain Mediates Immune Evasion
Figure 1
An active site affinity tag identifies processed active proteases of wild type and cruzain deficient T. cruzi.
Iodinated DCGO4 (0.1, 1, 10, and-50 µM) was reacted with epimastigote extracts (105 epimastigotes/lane). Processed, active cruzain (47.5 kD) was identified as the major protease in wild type T. cruzi. Higher molecular species identified as unprocessed cruzain by MALDI but no active protease were observed in cruzain-deficient parasites at 50 µM DCG04.