An Interaction between KSHV ORF57 and UIF Provides mRNA-Adaptor Redundancy in Herpesvirus Intronless mRNA Export
Figure 5
ORF57 is linked to the hTREX complex by UIF; however, ORF57 preferentially interacts with Aly over UIF.
(A) Recombinant GST, and GST-ORF57 were bound to glutathione agarose beads and GST Pull-down assays performed using purified recombinant UIF-His or control ORF73-His proteins. Precipitated proteins and inputs were analysed by western blotting using a His-specific antibody. (B) Reconstitutive GST pulldowns were performed using GST or GST-UAP56 bound to glutathione agarose beads and incubated with recombinant purified ORF57-His or Aly-His alone or in combination. Precipitated proteins and inputs were analysed by western blotting using a His-specific antibody.