An Interaction between KSHV ORF57 and UIF Provides mRNA-Adaptor Redundancy in Herpesvirus Intronless mRNA Export
Figure 3
KSHV ORF57 colocalises with UIF in the nucleus and nucleolus.
293T cells were either mock (Ai, Ci), or transfected with pORF57-mCherry (Aii, Cii) or pUIF-GFP (Bi, Di) and in combination (Bii, Dii), incubated for 24 h, fixed and immunofluoresence staining performed. ORF57 and UIF were visualised by direct fluorescence of Cherry and GFP, respectively. Subcellular localisation within the nuclear speckles or nucleolus was confirmed using antibodies specific to SC-35 (A, B) or B23 (C, D), respectively. A merge of the mCherry/GFP channels is also included for all images. White arrows indicate nuclear speckles (Bii) or nucleolar localisation (Dii).