Re-localization of Cellular Protein SRp20 during Poliovirus Infection: Bridging a Viral IRES to the Host Cell Translation Apparatus
Figure 5
SRp20 partial co-localization with PCBP2 in the cytoplasm of poliovirus-infected SK-N-SH cells.
Cells were transfected with GFP-SRp20 and mock-infected (A) or infected with poliovirus for 3 hours (B) at an MOI of 25. At 3 hours post-infection, cells were fixed and incubated with a PCBP2 monoclonal antibody, a secondary antibody conjugated to biotin, and streptavidin conjugated to Texas Red. Cells were examined for co-localization of PCBP2 and SRp20 (shown in the merged image in yellow and highlighted by the white arrow) in the cytoplasm of the cells using confocal microscopy; nuclei were identified by DAPI staining.