Unique Cell Adhesion and Invasion Properties of Yersinia enterocolitica O:3, the Most Frequent Cause of Human Yersiniosis
Figure 5
Expression analysis of Y. enterocolitica O:3 invasin, YadA, and RovA.
Y. enterocolitica O:3 strains and the serotype O:8 reference strain 8081v were grown overnight at 25°C (A) and 37°C (B). Whole cell extracts for analysis of the DNA-binding protein RovA and the adhesins InvA and YadA were prepared, separated on SDS-polyacrylamid gels and analyzed by western blotting using polyclonal antibodies directed against RovA, InvA and YadA. A molecular marker the PageRuler Prestained Protein Ladder was loaded on the left.