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Rotavirus Stimulates Release of Serotonin (5-HT) from Human Enterochromaffin Cells and Activates Brain Structures Involved in Nausea and Vomiting

Figure 2

NSP4 induces 5-HT release in a dose- and time-dependent manner.

Primary EC t.c. were incubated with recombinant NSP4 (n = 4) at different concentrations and the 5-HT concentration in the cell medium (n = 4) was determined at different time points by HPLC. The asterisk (*) denotes statistical significance (P<0.05; Mann Whitney test).

Figure 2

doi: https://doi.org/10.1371/journal.ppat.1002115.g002