Tipping the Balance: Sclerotinia sclerotiorum Secreted Oxalic Acid Suppresses Host Defenses by Manipulating the Host Redox Environment
Figure 2
Wild-type Sclerotinia, but not the OA-deficient (A2) mutant, reduces the host cellular environment.
Transgenic Nicotiana benthamiana leaves containing the redox sensitive GFP (roGFP) cassette were investigated by confocal microscopy using (A) 410 nm and (B) 474 nm filters for observation of roGFP under oxidizing and reducing conditions, respectively. Two lines were chosen (line 2 shown here) for further analysis and inoculated with agar plugs containing actively growing (C) Wild-type Sclerotinia, (D) oxalate deficient A2 mutant, (E) nox1 mutant, (F) nox2 mutant, (G) sod mutant, and (H) wild-type Botrytis cinerea. Eight hours post-inoculation leaves were visualized under a 474 nm filter for observation of the reduced form of the roGFP.