The Binding of Triclosan to SmeT, the Repressor of the Multidrug Efflux Pump SmeDEF, Induces Antibiotic Resistance in Stenotrophomonas maltophilia
Figure 2
Triclosan breaks the SmeT-DNA complex.
The γ-[32P] labeled 30 bp operator DNA (2 nM, 10000 cpm) of SmeT was incubated with 0.2 µM SmeT for 20 min at room temperature (lane 1). Subsequently, increasing concentrations (0.1 mM and 0.2 mM) of triclosan were added and the mixture further incubated at room temperature for 15 min more. Retarded complexes were separated in a 6% non-denaturing polyacrylamide gel. The positions of free DNA and the retarded SmeT-DNA complex are indicated with arrows.