13C Metabolic Flux Analysis Identifies an Unusual Route for Pyruvate Dissimilation in Mycobacteria which Requires Isocitrate Lyase and Carbon Dioxide Fixation
Figure 7
Metabolic flux map B for M. bovis BCG in glycerol limited continuous culture at slow growth rate (td = 69 h).
Flux values were normalized to the specific glycerol uptake rate which was arbitrarily given the value of 100. Mass spectral data of proteinogenic amino acids from steady state chemostat cultures grown on a mixture of 20% [U-13C] glycerol and 80% unlabeled glycerol and the physiological data of Table 1 were used to generate the two alternative flux estimations. Arrows are pointing in the net flux direction and the width of each line is proportional to the underlying flux value. Numerical values of estimated fluxes (Table S5) are indicated on each flux arrow. The abbreviations are as in Figure 4.