13C Metabolic Flux Analysis Identifies an Unusual Route for Pyruvate Dissimilation in Mycobacteria which Requires Isocitrate Lyase and Carbon Dioxide Fixation
Figure 3
Continuous culture of M. bovis BCG and ▵icl1 at a dilution rate of 0.03 h−1 (td = 23.1 h).
A 10% late log phase culture of M. bovis BCG (black line) or ▵icl1 (red line) was inoculated into the bioreactor. The cultures were operated as batch until day 4. Continuous culture was then started at a dilution rate of 0.03 h−1. Samples were removed and assayed for biomass (circles). CO2 production in the waste gas was measured using a dual tandem sensor gas analyser (squares). The results are representative of two experiments.