13C Metabolic Flux Analysis Identifies an Unusual Route for Pyruvate Dissimilation in Mycobacteria which Requires Isocitrate Lyase and Carbon Dioxide Fixation
Figure 2
Continuous culture of M. bovis BCG and ▵icl1 at a dilution rate of 0.01 h−1 (td = 69.3 h).
A 10% late log phase culture of M. bovis BCG (A) and ▵icl1 (B) was inoculated into the bioreactor. The cultures were operated as batch until day 4. Continuous culture was then started at a dilution rate of 0.01 h−1 Samples were removed and assayed for biomass (red line). CO2 production in the waste gas was measured using a dual tandem sensor gas analyser (green line) and filtered supernatants were assayed for glycerol (blue line). The results are representative of two experiments.