Coronavirus Gene 7 Counteracts Host Defenses and Modulates Virus Virulence
Figure 5
(A) Total RNA extracted from infected ST cells, at indicated times post infection, was analyzed using a Bioanalyzer. The position of 28S and 18S rRNAs are indicated. (B) 28S rRNA integrity. Graph of 28S fluorescence intensity, as measured by Bioanalyzer, in the RNA samples from ST cells infected with rTGEV-wt (blue) or rTGEV-Δ7 (red), collected at different times post infection. f.u., fluorescence units. Error bars indicate the standard deviation from three independent experiments. *, p-value <0.05. (C) ST cells were treated with caspase inhibitor ZVAD, and infected. Total RNA was extracted and analyzed using a Bioanalyzer. (D) ST cells were transfected with Poly(I:C), and total RNA was extracted 16 hours post transfection. ST cells were also infected with a vaccinia virus expressing T7 polymerase (T7), or with the vaccinia expressing T7 polymerase, and two additional vaccinia viruses expressing 2′-5′ OAS and RNase L (RL+OAS). Total RNA was extracted 24 hpi. In all cases, cell RNA integrity was analyzed using a Bioanalyzer.